Journal: Scientific Reports

Article Title: A 1-minute blood test detects decreased immune function and increased clinical risk in COVID-19 patients

doi: 10.1038/s41598-021-02863-2

Figure Lengend Snippet: ( A ) Kinetic interaction of AuNP with IgG subclasses indicative of type 1 immunity, including bovine IgG2, human IgG1, human IgG3, and mouse IgG2a. ( B ) Kinetic interaction of AuNP with type 2 immunity-related and other IgG subclasses, including bovine IgG1, human IgG2, human IgG4, mouse IgG1, mouse IgG2b, and mouse IgG3. Kinetic curves shown here are representative of multiple measurements. Graph ( A ) and ( B ) are presented at the same scale for direct comparison.

Article Snippet: The study of the interaction between the AuNP reagents and IgG subclasses from bovine, human and murine was conducted using the following materials: Bovine IgG1 (pep003, Bio-Rad, 1 mg/mL); bovine IgG2 (pep004, Bio-Rad, 1 mg/mL); human IgG1 (ab90283, Abcam, 3 mg/mL); human IgG2 (ab90284, Abcam, 2.2 mg/mL); human IgG3 (ab118462, Abcam, 2.2 mg/mL); human IgG4 (ab183266, Abcam, 1.5 mg/mL); mouse IgG1 (02-6100, Thermofisher, 1 mg/mL); mouse IgG2a (02-6200, Thermofisher, 1 mg/mL); mouse IgG2b (02-6300, Thermofisher, 1 mg/mL); mouse IgG3 (IMG5119A, Novus Biological, 0.5 mg/mL).

Techniques: Comparison

Journal: Scientific Reports

Article Title: A 1-minute blood test detects decreased immune function and increased clinical risk in COVID-19 patients

doi: 10.1038/s41598-021-02863-2

Figure Lengend Snippet: Correlation between D2Dx test scores and anti-SARS-CoV-2 IgG antibody OD values. The IgG value is expressed as the average OD determined by ELISA.

Article Snippet: The study of the interaction between the AuNP reagents and IgG subclasses from bovine, human and murine was conducted using the following materials: Bovine IgG1 (pep003, Bio-Rad, 1 mg/mL); bovine IgG2 (pep004, Bio-Rad, 1 mg/mL); human IgG1 (ab90283, Abcam, 3 mg/mL); human IgG2 (ab90284, Abcam, 2.2 mg/mL); human IgG3 (ab118462, Abcam, 2.2 mg/mL); human IgG4 (ab183266, Abcam, 1.5 mg/mL); mouse IgG1 (02-6100, Thermofisher, 1 mg/mL); mouse IgG2a (02-6200, Thermofisher, 1 mg/mL); mouse IgG2b (02-6300, Thermofisher, 1 mg/mL); mouse IgG3 (IMG5119A, Novus Biological, 0.5 mg/mL).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Dual-specificity phosphatase 14 (DUSP14/MKP6) negatively regulates TCR signaling by inhibiting TAB1 activation.

doi: 10.4049/jimmunol.1300989

Figure Lengend Snippet: FIGURE 7. Enhanced in vivo immune responses in DUSP14-deficient (DUSP14-KO) mice. WT and DUSP14-KO mice were immunized with KLH emulsified in alum. Seven days later, enlarged lymph nodes were isolated and restimulated with KLH for 72 h. (A) Cell proliferation was measured using [3H]thymidine incorporation. (B) IL-2, IL-4, and IFN-g levels in supernatants were measured using ELISA. Three mice were an- alyzed for each genotype. Data are mean 6 SEM. (C) WT and DUSP14- KO mice were immunized as before. Sera were collected at 14 d after the primary immunization. NP-specific IgM, IgG1, IgG2a, IgG2b, and IgG3 Abs were determined by ELISA. Results are presented relative to those of normal serum from a WT mouse. Five mice were analyzed for each ge- notype. Data are mean 6 SEM. *p , 0.05, two-tailed t test.

Article Snippet: The titers of anti–NP-specific IgM, IgG1, IgG2a, IgG2b, and IgG3 in the sera were measured by ELISAs (eBioscience) using NP-BSA (Biosearch Technologies) as the coating Ag, followed by incubation with anti-IgM, anti-IgG1, anti-IgG2a, anti-IgG2b, and anti-IgG3 goat anti-mouse Abs (all from Bethyl Laboratories) (19).

Techniques: In Vivo, Isolation, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: The Journal of Biological Chemistry

Article Title: ?-Arrestin Recruitment and G Protein Signaling by the Atypical Human Chemokine Decoy Receptor CCX-CKR *

doi: 10.1074/jbc.M112.406108

Figure Lengend Snippet: CCX-CKR internalizes CCL19. A, HEK293T cells transiently transfected with CCX-CKR, CCR7, or empty plasmid (mock) were incubated with 125I-CCL19 for the indicated time. Surface bound 125I-CCL19 was removed by an acid wash, and internalized 125I-CCL19 was quantified. B, U2OS osteosarcoma cells expressing β-arrestin2 fused to green fluorescent protein (U2OS-β-arr2-GFP) were stably transfected with vector coding for CCX-CKR (U2OS-CCX-CKR cells). U2OS-CCX-CKR cells were treated with Alexa Fluor 647-coupled CCL19 (CCL19-AF) at 37 (upper panels) or 4 °C (lower panels) for 45 min. Then, cells were washed with an acidic buffer (0.2 m glycine (pH 3.0), 0.5 m NaCl) or PBS for 5 min before fixation and staining. Alexa Fluor 647 and Hoechst signals are depicted in red and blue, respectively.

Article Snippet: Anti-mouse IgG-Alexa Fluor 647 and anti-rat IgG-Alexa Fluor 647 were purchased from R&D Systems.

Techniques: Transfection, Plasmid Preparation, Incubation, Expressing, Stable Transfection, Staining

Journal: The Journal of Biological Chemistry

Article Title: ?-Arrestin Recruitment and G Protein Signaling by the Atypical Human Chemokine Decoy Receptor CCX-CKR *

doi: 10.1074/jbc.M112.406108

Figure Lengend Snippet: Internalized CCL19 and β-arrestin co-localize in endocytic vesicles in cells expressing CCX-CKR. A, U2OS-β-arr2-GFP and U2OS-CCX-CKR cells were treated with 100 nm CCL19 for 45 min. Cells were then fixed, stained with Hoechst, and imaged using fluorescence microscopy for GFP (green) and nuclei (blue). B, U2OS-CCX-CKR cells were stimulated with increasing concentrations of CCL19, CCL21, and CCL25 for 45 min. The recruitment of β-arrestin2 to CCX-CKR was quantified as the amount of GFP-containing vesicles per nucleus. C, U2OS-CCX-CKR cells were treated with 100 nm CCL19 or CCL19-AF for 45 min, followed by fluorescence microscopy. GFP is shown in green, Alexa Fluor 647 is in red, and nuclei are stained blue. D, U2OS-CCX-CKR cells were treated with increasing doses of CCL19 and CCL19-AF for 45 min followed by determination of GFP-containing vesicle formation. E, quantification of AF-containing vesicle formation of U2OS-CCX-CKR cells treated with increasing concentrations of CCL19-AF.

Article Snippet: Anti-mouse IgG-Alexa Fluor 647 and anti-rat IgG-Alexa Fluor 647 were purchased from R&D Systems.

Techniques: Expressing, Staining, Fluorescence, Microscopy